MUSHROOMS THAT “BLEED,”

PRIMATES THAT FIND THEIR FOOD JUST WITH THEIR MIDDLE FINGER...

THESE ARE REAL THINGS, PEOPLE.

WE SHARE OUR PLANET WITH MILLIONS OF INCREDIBLE LIFE FORMS.

AND DISCOVERING WHO THEY ARE, WHERE THEY ARE, AND WHAT MAKES THEM TICK IS KEY IF WE WANT

TO UNLOCK THE SECRETS OF MEDICINE, ENGINEERING, DESIGN, ECONOMICS,

SUSTAINABILITY AND STEWARDSHIP

THAT WILL DEFINE THE FUTURE OF OUR SPECIES;

REVEAL CLUES ABOUT OUR EVOLUTIONARY PAST;

AND JUST CULTIVATE OUR SENSE OF WONDER AT LIFE'S SHEER POTENTIAL.

BUT WE NEED TO ACT FAST.

- Today we are living in a biodiversity crisis,

in which species are going extinct before we even realize their existence, or their

potential value.

SO, HOW CLOSE ARE WE TO DISCOVERING ALL LIFE ON EARTH?

IN THIS EPISODE, WE'LL MEET SOME AMAZING BIODIVERSITY RESEARCHERS

WORKING INTERNATIONALLY TO ACCELERATE SPECIES DISCOVERY,

DEVELOP DIGITAL CONSERVATION TOOLS,

AND MAINTAIN ONE OF THE WORLD'S LARGEST BIOLOGICAL COLLECTIONS

THROUGH A PROCESS CALLED “CRYOPRESERVATION”

THAT IS, LITERALLY, ULTRA-COOL.

BUT THIS NETWORK OF RESEARCHERS HAS A HERCULEAN EFFORT IN FRONT OF THEM.

- Strangely enough, we know only maybe 10% of the biodiversity that we have on our planet.

THAT MEANS THERE'S SOMETHING LIKE 90% OF LIFE ON EARTH LEFT TO DISCOVER.

SO LET'S START AT THE BEGINNING.

DESCRIBING A NEW SPECIES TYPICALLY REQUIRES COLLECTING A PHYSICAL SPECIMEN OF SOME KIND,

WHICH CAN OFTEN MEAN TRAVELING TO HARD-TO-REACH LOCATIONS.

- They call them these ”rapid inventories.” Sometimes they don't have road access so they'll

fly in by plane and just parachute in with a team of ecologists that identify the plants,

the birds, the amphibians and the butterflies.

- If a species is bigger, it's easier to find

than a small specimen.

If the species has a really colorful pattern, it's easier to see than a species that has

a kind of camouflage.

ONCE IT'S RETRIEVED, THE SAMPLE WILL BE SENT TO A SCIENTIFIC COLLECTION, WHERE IT

MAY HAVE TO WAIT TO BE  DISCOVERED ALL OVER AGAIN.

After you collect this specimen in nature,

it might take, like, 21 years for that specimen to be discovered in a scientific collection,

and have someone process it and describe it as a formal new species.

THIS IS BECAUSE THE EXPERTISE REQUIRED TO DO THIS IS PRETTY RARE.

A TAXONOMIC EXPERT NEEDS TO STUDY THE SPECIES' SHAPE, STRUCTURE, AND GENETIC MAKEUP, AND

COMPARE IT WITH ANY CLOSE KNOWN RELATIVES BEFORE GIVING IT A NAME.

We don't have enough taxonomists.

We need to have an army of taxonomists to describe all the species

that exist in the world.

BUT DUBBING A SPECIES OFFICIAL IS JUST THE BEGINNING.

TO CREATE A RECORD OF IT THAT OTHER SCIENTISTS CAN STUDY, LET ALONE USE FOR ANY CONSERVATION

WORK, WE NEED TO CAPTURE AND PRESERVE ALL THE INFORMATION WE CAN ABOUT AN ORGANISM.

THAT'S WHERE COLLECTIONS LIKE THE SMITHSONIAN

INSTITUTION'S BIOREPOSITORIES COME IN.

SO WE WENT TO VISIT THE NATIONAL ZOO IN DOWNTOWN WASHINGTON, D.C.

TO SEE WHAT HAPPENS NEXT.

- A biorepository is a specialized facility

that really contains a lot of biological materials,

that really need to be preserved in a stable state.

It's like in a museum when you have paintings that are very sensitive to variation of humidity

or temperature.

Every day, we have tens to hundreds of incoming samples

that are processed and stored for the long term.

I would say that we are probably among the largest collections in the world.

THAT COLLECTION REPRESENTS AROUND 18,000 SPECIES

AND INCLUDES OVER 1 MILLION DNA SAMPLES, SKIN

BIOPSIES, BLOOD SAMPLES, SPERM, EGGS, EMBRYOS...

YOU NAME IT.

CRYOPRESERVATION INVOLVES A SERIES OF METICULOUS STEPS,

WHICH I GOT TO EXPERIENCE FIRSTHAND.

THE WATER IN A SKIN SAMPLE FROM A NEWLY DISCOVERED RODENT,

FOR EXAMPLE, NEEDS TO BE REPLACED

WITH A FLUID CALLED CRYOPROTECTANT THAT ACTS AS ANTIFREEZE, EFFECTIVELY PUTTING THE CELLS

ON PAUSE WHEN THE SAMPLE IS STORED IN LIQUID NITROGEN.

So basically you suspend life entirely and

this life suspension can travel through time - for a long, long period of time.

- Do you mind if I try? Is that okay?

- Sure, go for it.

- You have to show me how to do it, though, because I have a feeling this is harder than it looks.

- A little.

- Okay. So, we've got the… things… and the goal is to put it in the tube, right? Okay.

- Mm-hmm, and while keeping everything underneath the liquid nitrogen,

as much as possible.

- Haha, I don't know if I'm going to be able to do that, okay.

Let's try.

Ah! Okay, okay. Like this... it's not submerged, it's not submerged… oh no.

Ha!

It's good, right?

- Nice!

Yaaaaaaas. High five.

We preserve also, all the packaging of the DNA but

we also preserve the cells that are containing the DNA and beyond the cells, the whole tissue.

We want to make sure that everything is still viable when we are thawing the samples.

Viable means that the tissue has to be able to recover and to resume normal activities,

biochemical, biophysical activities and when we are talking about gonadal tissues like

ovaries or testes, we need to make sure that they are still producing germinal cells.

YOU HEARD THAT RIGHT – THESE SAMPLES CAN BE USED

NOT ONLY FOR FUTURE SCIENTIFIC STUDY, BUT EVEN ARTIFICIAL INSEMINATION

TO HELP RECOVER AN ENDANGERED POPULATION.

AND WHILE THE ZOO ITSELF SPECIALIZES IN VERTEBRATES AND CARNIVORES,

THROUGH SOMETHING CALLED THE “PAN-SMITHSONIAN CRYOINITIATIVE,”

PIERRE AND HIS TEAM WORK WITH SCIENTISTS AT

OTHER ZOOS, MUSEUMS, AND RESEARCH CENTERS TO PRESERVE INVERTEBRATES, PLANTS, AND SOIL

SAMPLES, ADDING MICROBES AND FUNGI TO A MORE AND MORE COMPLETE 'LIBRARY OF LIFE.'

- So, there are specific parts of the planet that

we've barely explored, and there are specific parts of the tree of life, like specific animal

groups, that we haven't scientifically explored in the same way.

SO HOW DO WE KNOW WHERE TO LOOK NEXT?

WHERE MIGHT BE MOST AT RISK, OR MOST RIPE FOR DISCOVERIES?

TO CREATE A ROADMAP THAT POINTS US TO WHERE NEW SPECIES MIGHT BE HIDING, WE'LL NEED

TO COMPILE EVERYTHING WE KNOW SO FAR ABOUT WHERE SPECIES OCCUR,

FROM CONSERVATION DATASETS,

TO VINTAGE FIELD GUIDES, TO INFORMATION COLLECTED BY CITIZEN SCIENTISTS

ON THEIR MOBILE PHONES.

ENTER THE “MAP OF LIFE,” A FLAGSHIP PROJECT

AT YALE'S CENTER FOR BIODIVERSITY AND GLOBAL CHANGE THAT'S DOING JUST THAT.

What we do is compile all types of spatial and non-spatial information

about species, to get the best idea of where species occur in the world.

Our data teams can standardize millions of records so that we can really easily and

quickly get an idea of, 'this is what information we have for this specific region.'

AS THE NAME SUGGESTS, THIS DATA, TAKEN TOGETHER,

CREATES A VIRTUAL MAP OF LIFE THAT CAN BE USED BY ANYONE ,

WHETHER YOU'RE DISCOVERING SPECIES IN YOUR OWN BACKYARD

OR PLANNING A FIELD EXPEDITION.

We also inform UN-level decision makers, and conservation managers

that are working on the ground.

It gives an answer to people that are curious to say like, "how can I get involved?

How can I help?"

And this is showing like, "This is what you're stewarding in your area that you live."

Anywhere on the planet.

BUT WHETHER RACING TO FREEZE TISSUE SAMPLES THAT IMMORTALIZE A SPECIES

OR DRAWING DIGITAL MAPS THAT POINT US TO NEW DISCOVERIES,

RESEARCHERS AGREE THAT OUR BEST BET TO PRESERVE AND DOCUMENT

LIFE AS QUICKLY AS WE CAN, IS TO ACT LOCALLY AND THINK GLOBALLY.

We built a dashboard for Peru, Ecuador, and Columbia

for park managers there to figure out what species are in their park, what do

we expect to be there, and also what has been actually recorded there.

In some cases, a species only occurs in one smaller reserve, and nowhere else on the planet.

And many times the park manager doesn't know that.

So it's making these really important linkages for them to be able to make decisions at a

really small scale.

- We've been working extensively with Thailand,

with Vietnam.

We've been working with countries like Kuwait in the Middle East.

To build capacity and to help countries to build their own national gene bank

to make sure that they preserve their own biodiversity.

- The regions with the most rewarding potential to have new discoveries are the tropical forests.

They harbor most of the undescribed species on Earth,

amphibians, reptiles, birds, and mammals.

SO, IF ALL WE NEED TO DO IS PRESERVE  OUR TROPICAL FORESTS,

RECRUIT AN ARMY OF TAXONOMISTS TO EXPLORE THEIR LIVING TREASURES,

CLEAN OUT THE SHELVES OF OUR SCIENTIFIC COLLECTIONS

TO SEE WHAT MIGHT BE HIDING IN PLAIN SIGHT,

AND POUR MORE RESOURCES INTO PRESERVING AND PROCESSING

MILLIONS OF SAMPLES AND DIGITAL RECORDS TO BUILD A LIBRARY OF LIFE AND A ROAD

MAP THAT POINTS US TO NEW DISCOVERIES,

HOW CLOSE ARE WE TO DISCOVERING ALL LIFE ON EARTH?

-  In birds, in mammals, we have a better knowledge.

In amphibians and reptiles, that knowledge is not that good, but it's something at least.

But for invertebrates and marine organisms, that knowledge is really sparse.

At the current rate of discoveries it might

well take 50 years to describe all vertebrates.

So the other groups, like invertebrates or marine organisms -

I would say that it will take perhaps another 100 years.

Or more.

- When you think that there are millions of species on the planet

and when I tell you that we only have 18,000 in the biorepositories

at the Smithsonian, you can see that it's a very small proportion.

But when you think about it, I mean, everything grows exponentially.

Because 100 years ago, we had no idea what cryobiology was.

Ten years ago, we knew that we could sequence DNA but it was a very, very long process

and now it can be done in an hour for a single genome.

It's a transgenerational, multigenerational effort  and I think it's pretty encouraging

to know that there are good chances for those efforts to be carried on, and probably improve,

and go way faster than what we can do right now.

- When you're working in biodiversity conservation,

it's easy to lose hope because you're just seeing we're in a mass extinction crisis.

But we're in this age where technology can really change the landscape of how we operate,

in positive ways.

So I have a lot of hope that it's not too late,

and now is as good a time as any to do something about it.

For a complete guide to all things science, subscribe to Seeker.

And let us know in the comments below what we should investigate next.

This was actually a really special episode for me, because it was my first time

getting to travel with Seeker, go in a lab, and investigate

what the scientists were doing in real-time, so if you liked that

please leave it in the comments below, thumbs up and share this video.

Thanks so much for watching. Bye!