Name: 羅傑-比奇（丹佛斯中心）第1部分：植物病毒感染的生物學問題。 (Roger Beachy (Danforth Center) Part 1: Biology of Plant Virus Infection)
Uploaded: 2021-01-14T06:00:20.000Z
Duration: 28 min 2 s
Description: 【看影片學英語】數萬部 YouTube 影片，搭配英漢字典即點即查，輕鬆掌握單字發音與用法，長久累積看電影不必再看字幕。學這些英文用法：武漢肺炎,新型冠狀病毒,新冠肺炎,COVID-19

Good morning, thanks for joining the session this morning.

I'm Roger Beachy, a plant virologist and I work in biotechnology as well,

I'm from the Donald Danforth Plant Science Center in St. Louis, Missouri,

we're a new, relatively new, non-for-profit research institution,

and I want to talk to you today about viruses and plants.

I'm going to tell you a little bit about how they replicate, but more importantly,

the cell biology of how they set up the virus replication factories

and how the ability of the virus to use host mechanisms to move from cell to cell eventually ends up in pathogenesis.

The second part of my lecture, the second lecture,

will be related to how to use that information through biotechnology to develop virus-resistant crops.

So, I want to tie these two together, and hopefully what I introduce you today, in this first lecture,

So, what I want to talk about, again, is the cell and molecular biology of virus infection, replication and pathogenicity.

This slide shows several examples of virus infected plants.

The disease that's shown here results after the virus infects perhaps a single cell, or multiple cells,

spreads from cell to cell and then moves from one leaf to the next,

And as you see, the examples I have here are for cassava, in Africa, in papaya, throughout the world,

and this is an example of a disease on a tomato plant that shows severely affected plants in the front,

and disease resistant plants in the back.

Again, the result here is a culmination of events that leads from infection of a single cell

and spreading throughout the leaf and then throughout the plant.

Throughout my lecture, I'm going to talk primarily about tobacco mosaic virus.

It's a very simple plant virus, it's been used as a model for more than fifty years,

and scientists throughout the world have used it for a variety of studies.

I'm going to talk about it in terms of its cell biology and molecular biology of replication and spread.

There are lots of different examples of viruses in this category,

some in fact have hosts like tomato and peppers, but others have been identified that infect Arabidopsis and other model plants.

The genome of tobacco mosaic virus is a single, very simple RNA molecule.

It encodes three genes. The first is an enzyme that helps the virus copy itself, it's an RNA dependent RNA polymerase.

The green box indicates the protein that's encoded by the virus to help it to move from cell to cell,

and the blue box indicates the location of the capsid protein.

The capsid, of course, its role is to replicate, is to encapsulate the single-stranded genome

and allow it to be transmitted from cell to cell, or from leaf to leaf,

and of course, that's what's transmitted as workers in the field propagate plants and move the virus around.

Now from the standpoint of molecular virology, this is a slide from Dr. Milton Zaitlin at Cornell University,

and you see that in the first iteration, a virus enters the cell, usually by  a wound,

either by an insect or a worker who might be working in the field,

transmitting the virus into a broken cell, and from there on, the molecular structures take over,

and processes take over, the virus is encoded, releasing the viral RNA, ribosomes translate it,

and those enzymes that are produced from the translation are responsible for copying the RNA

and building the virus replication factories to make more virions,

eventually ending up with more virus particles that are shown down there, down in the lower left hand corner.

Now, from a cell biologist's point of view, it's a very different story.

In this case, we've looked at how the virus enters a cell, and in the first iteration, the virion is dis-encapsidated

within a few seconds after it enters a cell.

Viral RNA is then moved into, on ribosomes, it's moved into the membranes that are perinuclear

and there it begins its translation and set up the first parts of the protein machinery that are necessary to produce more virions,

then moves, those membranes then move out into the cytosolic portion of the cell,

and set up larger factories, and continue to build them on endoplasmic reticula,

Those bodies, then, reproduce themselves, move around the cells, as I'll show you later,

and eventually, then move from cell to cell.

And the real challenge here is for the virus to use the cellular machinery to do all of these processes

Unlike animal viruses, plant viruses can't bud from their cell membrane and then be adsorbed by others

because plant cells are surrounded by very rigid cell walls.

These cell walls are penetrated by structures that are known as plasmodesmata.

This cartoon, developed by my colleague Bernie Epel, in Israel, shows a little bit of the structure of those plasmodesmata.

This represents a cell wall between two adjacent cells, that you see here and over here.

And going through that hole in the wall is endoplasmic reticulum.

Now, myosins and actins and other energy conducting structures

make modifications throughout the living part of the plant's life cycle

and allows this membrane to move from one cell to the next in a very metered way.

These structures maintain homeostasis. They're very small and only allow very small molecules to move through,

including ions and energy carrying materials.

If that's disrupted, of course, that changes the homeostatic nature of the leaf and it would be damaging.

There are in fact, during early parts of development and later parts of development,

you can see some changes in the structure of these plasmodesmata.

Here you see they're very much more rigid and they're bolstered by additional beta-1,3-glucans

and other molecules that give some rigidity to those structures.

So, the question is, how does a virus manage to squeeze through that structure?

And different viruses do it in different ways.

In this diagram, we've shown a diagram for how a set of viruses called geminiviruses

move from cell to cell. These are single-stranded DNA viruses, they replicate in the nucleus,

they come out to the cytoplasm, and one of the parts of the protein-coding encoded by the virus

moves it from the nucleus to the cytosol, and then another protein carries it all the way through the plasmodesmata,

Tobacco mosaic virus, by contrast, does it in a very different way.

There's an interaction between the movement protein, membranes and the cytoskeleton,

which carry these structures to, and through, the plasmodesmata.

And that's a subject of most of my talk this morning.

What we've done is use tobacco mosaic virus in a variety of ways.

We of course use the fluorescent protein green fluorescent protein as fusions to the movement protein,

sometimes as fusions to the code protein, to follow infection around the cell, and between cells.

In other cases, we've used simple confocal microscopy, coupled with immuno-localization assays, or procedures.

So, let's get into the nature of infection.

In this structure, in this study we've infected the virus into a protoplast,

these are cells from which we've taken the cell wall, and so that's why you see it as a round structure,

and we've identified the three different virus proteins with different colors.

The red represents the location of the virus replicase, using an antibody specific for the replicase.

The green represents the location of the movement protein, and the blue, the site of the coat protein.

Now, this is at about fourteen hours post-infection, and as that structure rotated,

you were able to see that some of these spots overlay each other.

What that should indicate to you is that they're in similar locations.

But as that went around, you know that at fourteen hours,

most of those structures were on the surface of the protoplast, on the outside.

In this case, now at 21 hours post-infection, now as you see this rotation,

you see that the green bodies are larger than they were in the first slide that I showed