pushups, so you can join me on the floor if you want.

Now, I'm not doing this to show off or anything. I'm actually doing this for science.

[pained grunt]

You see what happened there?

My arms moved, my shoulders moved, my back and stomach muscles moved, my heart pumped

blood to all those different places. Pretty neat, huh?

Well, it turns out that how we make and use energy is a lot like sports or other kinds

of exercise

It can be hard work and a little bit complicated but if you do it right, it can come with some

tremendous payoffs.

But unlike hitting a ball with a stick, it's so marvelously complicated and awesome that

we're still unraveling the mysteries of how it all works. And it all starts with a

marvelous molecule that is one of you best friends: ATP.

Today I'm talking about energy and the process our cells, and other animal cells, go through

to provide themselves with power.

Cellular respiration is how we derive energy from the food we eat--specifically from glucose,

since most of what we eat ends up as glucose.

Here's the chemical formula for one molecule of glucose [C6H12O6]. In order to turn this

glucose into energy, we're going to need to add some oxygen. Six molecules of it, to be exact.

Through cellular respiration, we're going to turn that glucose and oxygen into 6 molecules

of CO2, 6 molecules of water and some energy that we can use for doing all our push ups.

So that's all well and good, but here's the thing: We can't just use that energy

to run a marathon or something. First our bodies have to turn that energy into a really

specific form of stored energy called ATP, or adenosine triphosphate. You've heard

me talk about this before. People often refer to ATP as the "currency" of biological energy.

Think of it as an American dollar--it's what you need to do business in the U.S. You

can't just walk into Best Buy with a handful of Chinese yen or Indian rupees and expect

to be able to buy anything with them, even though they are technically money. Same goes

with energy: In order to be able to use it, our cells need energy to be transferred into

adenosine triphosphate to be able to grow, move, create electrical impulses in our nerves

and brains. Everything. A while back, for instance, we talked about how cells use ATP

to transport some kinds of materials in and out of its membranes; to jog your memory about

that you can watch it right here.

Now before we see how ATP is put together, let's look at how cells cash in on the energy

that's stashed in there.

Well, adenosine triphosphate is made up of an nitrogenous base called adenine with a

sugar called ribose and three phosphate groups attached to it:

Now one thing you need to know about these 3 phosphate groups is that they are super

uncomfortable sitting together in a row like that -- like 3 kids on the bus who hate each

other all sharing the same seat.

So, because the phosphate groups are such terrible company for each other, ATP is able

to do this this nifty trick where it shoots one of the phosphates groups off the end of

the seat, creating ADP, or adenosine diphosphate (because now there are just two kids sitting

on the bus seat). In this reaction, when the third jerk kid is kicked off the seat, energy

is released.

And since there are a lot of water molecules just floating around nearby, an OH pairing

-- that's called a hydroxide -- from some H2O comes over and takes the place of that

third phosphate group. And everybody is much happier.

By the way? When you use water to break down a compound like this, it's called hydrolysis

-- hydro for water and lysis, from the Greek word for "separate."

So now that you know how ATP is spent, let's see how it's minted -- nice and new -- by

cellular respiration.

Like I said, it all starts with oxygen and glucose. In fact, textbooks make a point of

saying that through cellular respiration, one molecule of glucose can yield a bit of

heat and 38 molecules of ATP. Now, it's worth noting that this number is kind of a best

case scenario. Usually it's more like 29-30 ATPs, but whatever -- people are still studying

this stuff, so let's stick with that 38 number.

Now cellular respiration isn't something that just happens all at once -- glucose is

transformed into ATPs over 3 separate stages: glycolysis, the Krebs Cycle, and the electron

transport chain. Traditionally these stages are described as coming one after the other,

but really everything in a cell is kinda happening all at the same time.

But let's start with the first step: glycolysis, or the breaking down of the glucose.

Glucose, of course, is a sugar--you know this because it's got an "ose" at the end

of it. And glycolysis is just the breaking up of glucose's 6 carbon ring into two 3-carbon

molecules called pyruvic acids or pyruvate molecules.

Now in order to explain how exactly glycolysis works, I'd need about an hour of your time,

and a giant cast of finger puppets each playing a different enzyme, and though it would pain

me to do it, I'd have to use words like phosphoglucoisomerase.

But one simple way of explaining it is this: If you wanna make money, you gotta spend money.

Glycolysis needs the investment of 2 ATPs in order to work, and in the end it generates

4 ATPs, for a net profit, if you will, of 2 ATPs.

In addition to those 4 ATPs, glycolysis also results in 2 pyruvates and 2 super-energy-rich

morsels called NADH, which are sort of the love-children of a B vitamin called NAD+ pairing

with energized electrons and a hydrogen to create storehouses of energy that will later

be tapped to make ATP.

To help us keep track of all of the awesome stuff we're making here, let's keep score?

So far we've created 2 molecules of ATP and 2 molecules of NADH, which will be used

to power more ATP production later.

Now, a word about oxygen. Like I mentioned, oxygen is necessary for the overall process

of cellular respiration. But not every stage of it. Glycolysis, for example, can take place

without oxygen, which makes it an anaerobic process.

In the absence of oxygen, the pyruvates formed through glycolysis get rerouted into a process

called fermentation. If there's no oxygen in the cell, it needs more of that NAD+ to

keep the glycolysis process going. So fermentation frees up some NAD+, which happens to create

some interesting by products.

For instance, in some organisms, like yeasts, the product of fermentation is ethyl alcohol,

which is the same thing as all of this lovely stuff. But luckily for our day-to-day productivity,

our muscles don't make alcohol when they don't get enough oxygen. If that were the

case, working out would make us drunk, which actually would be pretty awesome, but instead

of ethyl alcohol, they make lactic acid. Which is what makes you feel sore after that workout

that kicked your butt.

So, your muscles used up all the oxygen they had, and they had to kick into anaerobic respiration

in order to get the energy that they needed, and so you have all this lactic acid building

up in your muscle tissue.

Back to the score. Now we've made 2 molecules of ATP through glycolysis, but your cells

really need the oxygen in order to make the other 30-some molecules they need.

That's because the next two stages of cellular respiration -- the Krebs Cycle and the electron transport

chain, are both aerobic processes, which means they require oxygen.

And so we find ourselves at the next step in cellular respiration after glycolosis:

the Krebs Cycle.

So, while glycolysis occurs in the cytoplasm, or the fluid medium within the cell that all

the organelles hang out in, the Krebs Cycle happens across the inner membrane of the mitochondria,

which are generally considered the power centers of the cell. The Krebs Cycle takes the products

of glycolysis -- those carbon-rich pyruvates -- and reworks them to create another 2 ATPs

per glucose molecule, plus some energy in a couple of other forms, which I'll talk

about in a minute. Here's how:

First, one of the pyruvates is oxidized, which basically means it's combined with oxygen.

One of the carbons off the three-carbon chain bonds with an oxygen molecule and leaves the

cell as CO2. What's left is a two-carbon compound called acetyl coenzyme A, or acetyl

coA. Then, another NAD+ comes along, picks up a hydrogen and becomes NADH. So our two

pyruvates create another 2 molecules of NADH to be used later.

As in glycolysis, and really all life, enzymes are essential here; they're proteins that

bring together the stuff that needs to react with each other, and they bring it together

in just the right way. These enzymes bring together a phosphate with ADP, to create another

ATP molecule for each pyruvate. Enzymes also help join the acetyl coA and a 4-carbon molecule

called oxaloacetic acid.

I think that's how you pronounce it.

Together they form a 6-carbon molecule called citric acid, and I'm certain that's how you

pronounce that one because that's the stuff that's in orange juice.

Fun fact: The Krebs Cycle is also known as the Citric Acid Cycle because of this very

byproduct. But it's usually referred to by the name of the man who figured it all out:

Hans Krebs, an ear nose and throat surgeon who fled Nazi Germany to teach biochemistry

at Cambridge, where he discovered this incredibly complex cycle in 1937. For being such a total

freaking genius, he was awarded the Nobel Prize in Medicine in 1953.

Anyway, the citric acid is then oxidized over a bunch of intricate steps, cutting carbons

off left and right, to eventually get back to oxaloacetic acid, which is what makes the

Krebs Cycle a cycle. And as the carbons get cleaved off the citric acid, there are leftovers

in the form of CO2 or carbon dioxide , which are exhaled by the cell, and eventually by

you. You and I, as we continue our existence as people, are exhaling the products of the

Krebs Cycle right now. Good work.

This video, by the way, I'm using a lot of ATPs making it.

Now, each time a carbon comes off the citric acid, some energy is made, but it's not

ATP. It's stored in a whole different kind of molecular package. This is where we go

back to NAD+ and its sort of colleague FAD.

NAD+ and FAD are both chummy little enzymes that are related to B vitamins, derivatives

of Niacin and Riboflavin, which you might have seen in the vitamin aisle. These B vitamins

are good at holding on to high energy electrons and keeping that energy until it can get released

later in the electron transport chain. In fact, they're so good at it that they show

up in a lot of those high energy-vitamin powders the kids are taking these days.

NAD+s and FADs are like batteries, big awkward batteries that pick up hydrogen and energized

electrons from each pyruvate, which in effect charges them up. The addition of hydrogen

turns them into NADH and FADH2, respectively.

Each pyruvate yeilds 3 NADHs and 1 FADH2 per cycle, and since each glucose has been broken

down into two pyruvates, that means each glucose molecule can produce 6 NADHs and 2 FADH2s.

The main purpose of the Krebs Cycle is to make these powerhouses for the next and final

step, the Electron Transport Chain.

And now's the time when you're saying, "Sweet pyruvate sandwiches, Hank, aren't we supposed

to be making ATP? Let's make it happen, Capt'n! What's the holdup?"

Well friends, your patience has paid off, because when it comes to ATPs, the electron

transport chain is the real moneymaker. In a very efficient cell, it can net a whopping

34 ATPs.

So, remember all those NADHs and FADH2s we made in the Krebs Cycle? Well, their electrons

are going to provide the energy that will work as a pump along a chain of channel proteins

across the inner membrane of the mitochondria where the Krebs Cycle occurred. These proteins

will swap these electrons to send hydrogen protons from inside the very center of the

mitochondria, across its inner membrane to the outer compartment of the mitochondria.

But once they're out, the protons want to get back to the other side of the inner membrane,

because there's a lot of other protons out there, and as we've learned, nature always

tends to seek a nice, peaceful balance on either side of a membrane. So all of these

anxious protons are allowed back in through a special protein called ATP synthase. And

the energy of this proton flow drives this crazy spinning mechanism that squeezes some

ADP and some phosphates together to form ATP. So, the electrons from the 10 NADHs that came

out of the Krebs Cycle have just enough energy to produce roughly 3 ATPs each.

And we can't forget our friends the FADH2s. We have two of them and they make 2 ATPs each.

And voila! That is how animal cells the world over make ATP through cellular respiration.

Now just to check, let's reset our ATP counter and do the math for a single glucose molecule

once again:

We made 2 ATPs for each pyruvate during glycolysis.

We made 2 in the Krebs Cycle.

And then during the electron transport chain we made about 34 in the electron transport chain.

And that's just for one molecule of glucose. Imagine how much your body makes and uses

every single day.

Don't spend it all in one place now! You can go back and watch any parts of this episode

that you didn't quite get and I really want to do this quickly because I'm getting very tired.

[Suck it up, Hank]

If you want to ask us questions you can see us in the YouTube comments below and of course,

you can connect with us on Facebook or Twitter.

[manly grunt]